Chemometric Methods for the Simultaneous Spectrophotometric Determination of Caffeine, Theobromine and Theophylline in Tea
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چکیده
منابع مشابه
Simultaneous HPLC determination of caffeine, theobromine, and theophylline in food, drinks, and herbal products.
A rapid and selective high-performance liquid chromatographic (HPLC) method is developed for the separation and determination of caffeine, theobromine, and theophylline. The chromatography is performed on a Zorbax Eclipse XDB-C8 column (4.6x150 mm i.d., 5-microm particle size) at 25 degrees C, with a mobile phase of water-THF (0.1% THF in water, pH 8)-acetonitrile (90:10, v/v). The flow rate is...
متن کاملSimultaneous determination of caffeine, theobromine and theophylline in foods and pharmaceutical preparations by using ion chromatography
Two ion chromatographic methods have been proposed for the simultaneous determination of caffeine, theobromine and theophylline. The separations were based on isocratic elutions and the determinations on ultraviolet absorbance detections at 274 nm. The detection limits (signal-to-noise ratio 3:1) for the three compounds were all below sub-mg mlÿ1 level. The methods have been successfully applie...
متن کاملSimultaneous determination of caffeine, theobromine, and theophylline by high-performance liquid chromatography.
This work relates the development of an analytical methodology to simultaneously determine three methylxanthines (caffeine, theobromine, and theophylline) in beverages and urine samples based on reversed-phase high-performance liquid chromatography. Separation is made with a Bondesil C18 column using methanol-water-acetic acid or ethanol-water-acetic acid (20:75:5, v/v/v) as the mobile phase at...
متن کاملSimple, Fast and Reliable Liquid Chromatographic and Spectrophotometric Methods for the Determination of Theophylline in Urine, Saliva and Plasma Samples
In this study, a high-performance liquid chromatographic method (HPLC) and UV spectrophotometric method were developed, validated and applied for the determination of theophylline in biological fluids. Liquid- liquid extraction is performed for isolation of the drug and elimination of plasma and saliva interferences. Urine samples were applied without any extraction. The chromatographic separat...
متن کاملSimple, Fast and Reliable Liquid Chromatographic and Spectrophotometric Methods for the Determination of Theophylline in Urine, Saliva and Plasma Samples
In this study, a high-performance liquid chromatographic method (HPLC) and UV spectrophotometric method were developed, validated and applied for the determination of theophylline in biological fluids. Liquid- liquid extraction is performed for isolation of the drug and elimination of plasma and saliva interferences. Urine samples were applied without any extraction. The chromatographic separat...
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ژورنال
عنوان ژورنال: Asian Journal of Chemistry
سال: 2013
ISSN: 0970-7077,0975-427X
DOI: 10.14233/ajchem.2013.14735